Characterization of spontaneously generated prion-like conformers in cultured cells
Identifieur interne : 000064 ( France/Analysis ); précédent : 000063; suivant : 000065Characterization of spontaneously generated prion-like conformers in cultured cells
Auteurs : Roger S. Zou [États-Unis] ; Hisashi Fujioka [États-Unis] ; Jian-Ping Guo [Canada] ; Xiangzhu Xiao [États-Unis] ; Miyuki Shimoji [États-Unis] ; Crystal Kong [États-Unis] ; Cecilia Chen [États-Unis] ; Megan Tasnadi [États-Unis] ; Chesinta Voma [États-Unis] ; Jue Yuan [États-Unis] ; Mohammed Moudjou [France] ; Hubert Laude [France] ; Robert B. Petersen [États-Unis] ; Wen-Quan Zou [États-Unis]Source :
- Aging (Albany NY) [ 1945-4589 ] ; 2011.
Abstract
A distinct conformational transition from the α-helix-rich cellular prion protein (PrPC) into its β-sheet-rich pathological isoform (PrPSc) is the hallmark of prion diseases, a group of fatal transmissible encephalopathies that includes spontaneous and acquired forms. Recently, a PrPSc-like intermediate form characterized by the formation of insoluble aggregates and protease-resistant PrP species termed insoluble PrPC (iPrPC) has been identified in uninfected mammalian brains and cultured neuronal cells, providing new insights into the molecular mechanism(s) of these diseases. Here, we explore the molecular characteristics of the spontaneously formed iPrPC in cultured neuroblastoma cells expressing wild-type or mutant human PrP linked to two familial prion diseases. We observed that although PrP mutation at either residue 183 from Thr to Ala (PrPT183A) or at residue 198 from Phe to Ser (PrPF198S) affects glycosylation at both N-linked glycosylation sites, the T183A mutation that results in intracellular retention significantly increased the formation of iPrPC. Moreover, while autophagy is increased in F198S cells, it was significantly decreased in T183A cells. Our results indicate that iPrPC may be formed more readily in an intracellular compartment and that a significant increase in PrPT183A aggregation may be attributable to the inhibition of autophagy.
Url:
PubMed: 21990137
PubMed Central: 3229973
Affiliations:
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PMC:3229973Le document en format XML
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Zou</name><affiliation wicri:level="1"><nlm:aff id="A1"> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 44106</wicri:noRegion></affiliation></author><author><name sortKey="Fujioka, Hisashi" sort="Fujioka, Hisashi" uniqKey="Fujioka H" first="Hisashi" last="Fujioka">Hisashi Fujioka</name><affiliation wicri:level="1"><nlm:aff id="A2"> Department of Pharmacology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Pharmacology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 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Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 44106</wicri:noRegion></affiliation></author><author><name sortKey="Voma, Chesinta" sort="Voma, Chesinta" uniqKey="Voma C" first="Chesinta" last="Voma">Chesinta Voma</name><affiliation wicri:level="1"><nlm:aff id="A1"> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 44106</wicri:noRegion></affiliation></author><author><name sortKey="Yuan, Jue" sort="Yuan, Jue" uniqKey="Yuan J" first="Jue" last="Yuan">Jue Yuan</name><affiliation wicri:level="1"><nlm:aff id="A1"> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 44106</wicri:noRegion></affiliation></author><author><name sortKey="Moudjou, Mohammed" sort="Moudjou, Mohammed" uniqKey="Moudjou M" first="Mohammed" last="Moudjou">Mohammed Moudjou</name><affiliation wicri:level="3"><nlm:aff id="A9"> Virologie Immunologie Moléculaires, UR892 INRA 78350 Jouy-en-Josas, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea> Virologie Immunologie Moléculaires, UR892 INRA 78350 Jouy-en-Josas</wicri:regionArea><placeName><region type="region" 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Petersen</name><affiliation wicri:level="1"><nlm:aff id="A1"> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 44106</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A3"> Department of Neurology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Neurology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 44106</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A4"> Department of Neuroscience, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Neuroscience, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 44106</wicri:noRegion></affiliation></author><author><name sortKey="Zou, Wen Quan" sort="Zou, Wen Quan" uniqKey="Zou W" first="Wen-Quan" last="Zou">Wen-Quan Zou</name><affiliation wicri:level="1"><nlm:aff id="A1"> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 44106</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A3"> Department of Neurology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Neurology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 44106</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A7"> National Prion Disease Pathology Surveillance Center, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> National Prion Disease Pathology Surveillance Center, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 44106</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">21990137</idno><idno type="pmc">3229973</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3229973</idno><idno type="RBID">PMC:3229973</idno><date when="2011">2011</date><idno type="wicri:Area/Pmc/Corpus">000798</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000798</idno><idno type="wicri:Area/Pmc/Curation">000798</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000798</idno><idno type="wicri:Area/Pmc/Checkpoint">000818</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000818</idno><idno type="wicri:Area/Ncbi/Merge">000287</idno><idno type="wicri:Area/Ncbi/Curation">000287</idno><idno type="wicri:Area/Ncbi/Checkpoint">000287</idno><idno type="wicri:Area/Main/Merge">001410</idno><idno type="wicri:Area/Main/Curation">001408</idno><idno type="wicri:Area/Main/Exploration">001408</idno><idno type="wicri:Area/France/Extraction">000064</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Characterization of spontaneously generated prion-like conformers in cultured cells</title><author><name sortKey="Zou, Roger S" sort="Zou, Roger S" uniqKey="Zou R" first="Roger S." last="Zou">Roger S. Zou</name><affiliation wicri:level="1"><nlm:aff id="A1"> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 44106</wicri:noRegion></affiliation></author><author><name sortKey="Fujioka, Hisashi" sort="Fujioka, Hisashi" uniqKey="Fujioka H" first="Hisashi" last="Fujioka">Hisashi Fujioka</name><affiliation wicri:level="1"><nlm:aff id="A2"> Department of Pharmacology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Pharmacology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 44106</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A5"> Electron Microscopy Facility, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Electron Microscopy Facility, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 44106</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A6"> Center for Mitochondrial Disease, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Center for Mitochondrial Disease, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 44106</wicri:noRegion></affiliation></author><author><name sortKey="Guo, Jian Ping" sort="Guo, Jian Ping" uniqKey="Guo J" first="Jian-Ping" last="Guo">Jian-Ping Guo</name><affiliation wicri:level="1"><nlm:aff id="A8"> Kinsmen Laboratory of Neurological Research, University of British Columbia, Vancouver, BC V6T 1Z3, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea> Kinsmen Laboratory of Neurological Research, University of British Columbia, Vancouver, BC V6T 1Z3</wicri:regionArea><wicri:noRegion>BC V6T 1Z3</wicri:noRegion></affiliation></author><author><name sortKey="Xiao, Xiangzhu" sort="Xiao, Xiangzhu" uniqKey="Xiao X" first="Xiangzhu" last="Xiao">Xiangzhu Xiao</name><affiliation wicri:level="1"><nlm:aff id="A1"> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 44106</wicri:noRegion></affiliation></author><author><name sortKey="Shimoji, Miyuki" sort="Shimoji, Miyuki" uniqKey="Shimoji M" first="Miyuki" last="Shimoji">Miyuki Shimoji</name><affiliation wicri:level="1"><nlm:aff id="A1"> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 44106</wicri:noRegion></affiliation></author><author><name sortKey="Kong, Crystal" sort="Kong, Crystal" uniqKey="Kong C" first="Crystal" last="Kong">Crystal Kong</name><affiliation wicri:level="1"><nlm:aff id="A1"> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 44106</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Cecilia" sort="Chen, Cecilia" uniqKey="Chen C" first="Cecilia" last="Chen">Cecilia Chen</name><affiliation wicri:level="1"><nlm:aff id="A1"> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 44106</wicri:noRegion></affiliation></author><author><name sortKey="Tasnadi, Megan" sort="Tasnadi, Megan" uniqKey="Tasnadi M" first="Megan" last="Tasnadi">Megan Tasnadi</name><affiliation wicri:level="1"><nlm:aff id="A1"> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 44106</wicri:noRegion></affiliation></author><author><name sortKey="Voma, Chesinta" sort="Voma, Chesinta" uniqKey="Voma C" first="Chesinta" last="Voma">Chesinta Voma</name><affiliation wicri:level="1"><nlm:aff id="A1"> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 44106</wicri:noRegion></affiliation></author><author><name sortKey="Yuan, Jue" sort="Yuan, Jue" uniqKey="Yuan J" first="Jue" last="Yuan">Jue Yuan</name><affiliation wicri:level="1"><nlm:aff id="A1"> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 44106</wicri:noRegion></affiliation></author><author><name sortKey="Moudjou, Mohammed" sort="Moudjou, Mohammed" uniqKey="Moudjou M" first="Mohammed" last="Moudjou">Mohammed Moudjou</name><affiliation wicri:level="3"><nlm:aff id="A9"> Virologie Immunologie Moléculaires, UR892 INRA 78350 Jouy-en-Josas, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea> Virologie Immunologie Moléculaires, UR892 INRA 78350 Jouy-en-Josas</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Jouy-en-Josas</settlement></placeName></affiliation></author><author><name sortKey="Laude, Hubert" sort="Laude, Hubert" uniqKey="Laude H" first="Hubert" last="Laude">Hubert Laude</name><affiliation wicri:level="3"><nlm:aff id="A9"> Virologie Immunologie Moléculaires, UR892 INRA 78350 Jouy-en-Josas, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea> Virologie Immunologie Moléculaires, UR892 INRA 78350 Jouy-en-Josas</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Jouy-en-Josas</settlement></placeName></affiliation></author><author><name sortKey="Petersen, Robert B" sort="Petersen, Robert B" uniqKey="Petersen R" first="Robert B." last="Petersen">Robert B. Petersen</name><affiliation wicri:level="1"><nlm:aff id="A1"> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 44106</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A3"> Department of Neurology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Neurology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 44106</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A4"> Department of Neuroscience, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Neuroscience, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 44106</wicri:noRegion></affiliation></author><author><name sortKey="Zou, Wen Quan" sort="Zou, Wen Quan" uniqKey="Zou W" first="Wen-Quan" last="Zou">Wen-Quan Zou</name><affiliation wicri:level="1"><nlm:aff id="A1"> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Pathology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 44106</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A3"> Department of Neurology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> Department of Neurology, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 44106</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A7"> National Prion Disease Pathology Surveillance Center, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea> National Prion Disease Pathology Surveillance Center, Case Western Reserve University School of Medicine, 2085 Adelbert Road, Cleveland, Ohio 44106</wicri:regionArea><wicri:noRegion>Ohio 44106</wicri:noRegion></affiliation></author></analytic><series><title level="j">Aging (Albany NY)</title><idno type="eISSN">1945-4589</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>A distinct conformational transition from the α-helix-rich cellular prion protein (PrP<sup>C</sup>) into its β-sheet-rich pathological isoform (PrP<sup>Sc</sup>) is the hallmark of prion diseases, a group of fatal transmissible encephalopathies that includes spontaneous and acquired forms. Recently, a PrP<sup>Sc</sup>-like intermediate form characterized by the formation of insoluble aggregates and protease-resistant PrP species termed insoluble PrP<sup>C</sup> (iPrP<sup>C</sup>) has been identified in uninfected mammalian brains and cultured neuronal cells, providing new insights into the molecular mechanism(s) of these diseases. Here, we explore the molecular characteristics of the spontaneously formed iPrP<sup>C</sup> in cultured neuroblastoma cells expressing wild-type or mutant human PrP linked to two familial prion diseases. We observed that although PrP mutation at either residue 183 from Thr to Ala (PrP<sup>T183A</sup>) or at residue 198 from Phe to Ser (PrP<sup>F198S</sup>) affects glycosylation at both N-linked glycosylation sites, the T183A mutation that results in intracellular retention significantly increased the formation of iPrP<sup>C</sup>. Moreover, while autophagy is increased in F198S cells, it was significantly decreased in T183A cells. Our results indicate that iPrP<sup>C</sup> may be formed more readily in an intracellular compartment and that a significant increase in PrP<sup>T183A</sup> aggregation may be attributable to the inhibition of autophagy.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Prusiner, Sb" uniqKey="Prusiner S">SB Prusiner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Aguzzi, A" uniqKey="Aguzzi A">A Aguzzi</name></author><author><name sortKey="Polymenidou, M" uniqKey="Polymenidou M">M Polymenidou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jarrett, Jt" uniqKey="Jarrett J">JT Jarrett</name></author><author><name sortKey="Lansbury, Pt" uniqKey="Lansbury P">PT Lansbury</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yuan, J" uniqKey="Yuan J">J Yuan</name></author><author><name sortKey="Xiao, X" uniqKey="Xiao X">X 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G" uniqKey="Perry G">G Perry</name></author><author><name sortKey="Kong, Q" uniqKey="Kong Q">Q Kong</name></author><author><name sortKey="Parchi, P" uniqKey="Parchi P">P Parchi</name></author><author><name sortKey="Tagliavini, F" uniqKey="Tagliavini F">F Tagliavini</name></author><author><name sortKey="Gambetti, P" uniqKey="Gambetti P">P Gambetti</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zou, Wq" uniqKey="Zou W">WQ Zou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zou, Wq" uniqKey="Zou W">WQ Zou</name></author><author><name sortKey="Xiao, X" uniqKey="Xiao X">X Xiao</name></author><author><name sortKey="Yuan, J" uniqKey="Yuan J">J Yuan</name></author><author><name sortKey="Puoti, G" uniqKey="Puoti G">G Puoti</name></author><author><name sortKey="Fujioka, H" uniqKey="Fujioka H">H Fujioka</name></author><author><name sortKey="Wang, X" uniqKey="Wang X">X Wang</name></author><author><name sortKey="Richardson, S" uniqKey="Richardson S">S Richardson</name></author><author><name sortKey="Zhou, X" uniqKey="Zhou X">X Zhou</name></author><author><name sortKey="Zou, R" uniqKey="Zou R">R Zou</name></author><author><name sortKey="Li, S" uniqKey="Li S">S Li</name></author><author><name sortKey="Zhu, X" uniqKey="Zhu X">X Zhu</name></author><author><name sortKey="Mcgeer, Pl" uniqKey="Mcgeer P">PL McGeer</name></author><author><name sortKey="Mcgeehan, J" uniqKey="Mcgeehan J">J McGeehan</name></author><author><name sortKey="Kneale, G" uniqKey="Kneale G">G Kneale</name></author><author><name sortKey="Rincon Limas, De" uniqKey="Rincon Limas D">DE Rincon-Limas</name></author><author><name sortKey="Fernandez Funez, P" uniqKey="Fernandez Funez P">P Fernandez-Funez</name></author><author><name sortKey="Lee, Hg" uniqKey="Lee H">HG Lee</name></author><author><name sortKey="Smith, Ma" uniqKey="Smith M">MA Smith</name></author><author><name sortKey="Petersen, Rb" uniqKey="Petersen R">RB Petersen</name></author><author><name sortKey="Guo, Jp" uniqKey="Guo J">JP Guo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zou, Wq" uniqKey="Zou W">WQ Zou</name></author><author><name sortKey="Zhou, X" uniqKey="Zhou X">X Zhou</name></author><author><name sortKey="Yuan, J" uniqKey="Yuan J">J Yuan</name></author><author><name sortKey="Xiao, X" uniqKey="Xiao X">X Xiao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guo, Jp" uniqKey="Guo J">JP Guo</name></author><author><name sortKey="Petric, M" uniqKey="Petric M">M Petric</name></author><author><name sortKey="Campbell, W" uniqKey="Campbell W">W Campbell</name></author><author><name sortKey="Mcgeer, Pl" uniqKey="Mcgeer P">PL McGeer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zou, Wq" uniqKey="Zou W">WQ Zou</name></author><author><name sortKey="Cashman, Nr" uniqKey="Cashman N">NR Cashman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hanaichi, T" uniqKey="Hanaichi T">T Hanaichi</name></author><author><name sortKey="Sato, T" uniqKey="Sato T">T Sato</name></author><author><name sortKey="Iwamoto, T" uniqKey="Iwamoto T">T Iwamoto</name></author><author><name sortKey="Malavasi Yamashiro, J" uniqKey="Malavasi Yamashiro J">J Malavasi-Yamashiro</name></author><author><name sortKey="Hoshino, M" uniqKey="Hoshino M">M Hoshino</name></author><author><name sortKey="Mizuno, N" uniqKey="Mizuno N">N Mizuno</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Canada</li><li>France</li><li>États-Unis</li></country><region><li>Île-de-France</li></region><settlement><li>Jouy-en-Josas</li></settlement></list><tree><country name="États-Unis"><noRegion><name sortKey="Zou, Roger S" sort="Zou, Roger S" uniqKey="Zou R" first="Roger S." last="Zou">Roger S. Zou</name></noRegion><name sortKey="Chen, Cecilia" sort="Chen, Cecilia" uniqKey="Chen C" first="Cecilia" last="Chen">Cecilia Chen</name><name sortKey="Fujioka, Hisashi" sort="Fujioka, Hisashi" uniqKey="Fujioka H" first="Hisashi" last="Fujioka">Hisashi Fujioka</name><name sortKey="Fujioka, Hisashi" sort="Fujioka, Hisashi" uniqKey="Fujioka H" first="Hisashi" last="Fujioka">Hisashi Fujioka</name><name sortKey="Fujioka, Hisashi" sort="Fujioka, Hisashi" uniqKey="Fujioka H" first="Hisashi" last="Fujioka">Hisashi Fujioka</name><name sortKey="Kong, Crystal" sort="Kong, Crystal" uniqKey="Kong C" first="Crystal" last="Kong">Crystal Kong</name><name sortKey="Petersen, Robert B" sort="Petersen, Robert B" uniqKey="Petersen R" first="Robert B." last="Petersen">Robert B. Petersen</name><name sortKey="Petersen, Robert B" sort="Petersen, Robert B" uniqKey="Petersen R" first="Robert B." last="Petersen">Robert B. Petersen</name><name sortKey="Petersen, Robert B" sort="Petersen, Robert B" uniqKey="Petersen R" first="Robert B." last="Petersen">Robert B. Petersen</name><name sortKey="Shimoji, Miyuki" sort="Shimoji, Miyuki" uniqKey="Shimoji M" first="Miyuki" last="Shimoji">Miyuki Shimoji</name><name sortKey="Tasnadi, Megan" sort="Tasnadi, Megan" uniqKey="Tasnadi M" first="Megan" last="Tasnadi">Megan Tasnadi</name><name sortKey="Voma, Chesinta" sort="Voma, Chesinta" uniqKey="Voma C" first="Chesinta" last="Voma">Chesinta Voma</name><name sortKey="Xiao, Xiangzhu" sort="Xiao, Xiangzhu" uniqKey="Xiao X" first="Xiangzhu" last="Xiao">Xiangzhu Xiao</name><name sortKey="Yuan, Jue" sort="Yuan, Jue" uniqKey="Yuan J" first="Jue" last="Yuan">Jue Yuan</name><name sortKey="Zou, Wen Quan" sort="Zou, Wen Quan" uniqKey="Zou W" first="Wen-Quan" last="Zou">Wen-Quan Zou</name><name sortKey="Zou, Wen Quan" sort="Zou, Wen Quan" uniqKey="Zou W" first="Wen-Quan" last="Zou">Wen-Quan Zou</name><name sortKey="Zou, Wen Quan" sort="Zou, Wen Quan" uniqKey="Zou W" first="Wen-Quan" last="Zou">Wen-Quan Zou</name></country><country name="Canada"><noRegion><name sortKey="Guo, Jian Ping" sort="Guo, Jian Ping" uniqKey="Guo J" first="Jian-Ping" last="Guo">Jian-Ping Guo</name></noRegion></country><country name="France"><region name="Île-de-France"><name sortKey="Moudjou, Mohammed" sort="Moudjou, Mohammed" uniqKey="Moudjou M" first="Mohammed" last="Moudjou">Mohammed Moudjou</name></region><name sortKey="Laude, Hubert" sort="Laude, Hubert" uniqKey="Laude H" first="Hubert" last="Laude">Hubert Laude</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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